MSI (Microsatellite Instability)
MSI is the detection and determination of levels of MicroSatellite Instability (MSI), which characterizes different subtypes of tumors in many cancers and is caused either by mutations or by epigenetic inactivation (hypermethylation of the gene promoter). MLH1) of the genes required to correct the MisMatch Repair genes (MMR). It is detected in ~ 15% of cases of stage II and III colon cancer (CRC).
Method: In DNA from whole blood and FFPE sections, the polymorphic regions are checked BAT25, BAT26, NR21, NR24, MONO27 which are characterized as almost monomorphic which makes them ideal indicators for MSI as well as polymorphic areas PentaD and PentaC to confirm the origin of the two materials from the same incident using the kit MSI v1.2®Promega followed by capillary electrophoresis in ABI3130xl genetic analyzer (ThermoScientific).
Referrals of EOPYY accepted